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Rabbit anti-DHX16 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
DHX16
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 991 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Country of Origin
USA

Additional Product Information

DHX16 is a member of the DEAH-box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH-box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DH16 was originally identified as DBP2 and discovered to be a functional homolog of the yeast RNA helicase and ATPase, Cdc28/Prp68. DHX16 appears to play a key role in nonsense-mediated mRNA decay (NMD) as well as rRNA processing.

8449
DHX16
DEAH-box helicase 16
O60231
Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16

Alternate Names

ATP-dependent RNA helicase #3; DBP2; DDX16; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 16; DEAD/H box 16; DEAH (Asp-Glu-Ala-His) box polypeptide 16; DEAH-box protein 16; pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16; PRO2014; Prp2; PRP8; PRPF2; putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16; RNA helicase

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.