Rabbit anti-DHX16 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
DHX16 is a member of the DEAH-box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH-box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DH16 was originally identified as DBP2 and discovered to be a functional homolog of the yeast RNA helicase and ATPase, Cdc28/Prp68. DHX16 appears to play a key role in nonsense-mediated mRNA decay (NMD) as well as rRNA processing.
Alternate Names
ATP-dependent RNA helicase #3; DBP2; DDX16; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 16; DEAD/H box 16; DEAH (Asp-Glu-Ala-His) box polypeptide 16; DEAH-box protein 16; pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16; PRO2014; Prp2; PRP8; PRPF2; putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16; RNA helicase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.