Rabbit anti-Dishevelled 2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-953A maps to a region between residue 650 and 700 of human Dishevelled, DSH Homolog 2 using the numbering given in entry NP_004413.1 (GeneID 1856).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Dishevelled 2 immobilized on solid support.
The epitope recognized by A302-953A-T maps to a region between residue 650 and 700 of human Dishevelled, DSH Homolog 2 using the numbering given in entry NP_004413.1 (GeneID 1856).
Additional Product Information
Dishevelled 2 is a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. Dishevelled 2 may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans [taken from NCBI Entrez Gene (Gene ID: 1856)].
Alternate Names
dishevelled 2 (homologous to Drosophila dsh); dishevelled, dsh homolog 2; Dishevelled-2; DSH homolog 2; segment polarity protein dishevelled homolog DVL-2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.