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Primary Antibodies  

Rabbit anti-DLD Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

DLD

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 459 and 509

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Dog

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Dihydrolipoamide dehydrogenase (DLD) is a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. DLD has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, DLD functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency [taken from NCBI Entrez Gene (Gene ID: 1738)].

1738

DLD

dihydrolipoamide dehydrogenase

P09622

Dihydrolipoyl dehydrogenase, mitochondrial

Alternate Names

diaphorase; Dihydrolipoamide dehydrogenase; dihydrolipoyl dehydrogenase, mitochondrial; DLDD; DLDH; E3; E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex; epididymis secretory sperm binding protein; GCSL; glycine cleavage system L protein; glycine cleavage system protein L; LAD; lipoamide dehydrogenase; lipoamide reductase; lipoyl dehydrogenase; PHE3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.