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Rabbit anti-E2-EPF Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
E2-EPF
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 172 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
X. laevis, X. tropicalis, Zebrafish, Bovine
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to E2-EPF immobilized on solid support.

The epitope recognized by A304-105A maps to a region between residue 172 to 222 of human Ubiquitin-Conjugating Enzyme E2S using the numbering given in entry NP_055316.2 (GeneID 27338).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to E2-EPF immobilized on solid support.

The epitope recognized by A304-105A-T maps to a region between residue 172 to 222 of human Ubiquitin-Conjugating Enzyme E2S using the numbering given in entry NP_055316.2 (GeneID 27338).
Country of Origin
USA

Additional Product Information

Ubiquitin-conjugating enzyme E2-EPF5 is a member of the ubiquitin-conjugating enzyme family. E2-EPF is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins [taken from NCBI Entrez Gene (Gene ID: 27338)].

27338
UBE2S
ubiquitin conjugating enzyme E2 S
Q16763
Ubiquitin-conjugating enzyme E2 S

Alternate Names

E2 ubiquitin-conjugating enzyme S; E2EPF; E2-EPF; E2-EPF5; EPF5; ubiquitin carrier protein S; ubiquitin conjugating enzyme E2S; ubiquitin-conjugating enzyme E2 S; ubiquitin-conjugating enzyme E2-24 kD; ubiquitin-conjugating enzyme E2-24 kDa; ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-protein ligase S

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.