Rabbit anti-E2-EPF Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A304-105A maps to a region between residue 172 to 222 of human Ubiquitin-Conjugating Enzyme E2S using the numbering given in entry NP_055316.2 (GeneID 27338).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to E2-EPF immobilized on solid support.
The epitope recognized by A304-105A-T maps to a region between residue 172 to 222 of human Ubiquitin-Conjugating Enzyme E2S using the numbering given in entry NP_055316.2 (GeneID 27338).
Additional Product Information
Ubiquitin-conjugating enzyme E2-EPF5 is a member of the ubiquitin-conjugating enzyme family. E2-EPF is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins [taken from NCBI Entrez Gene (Gene ID: 27338)].
Alternate Names
E2 ubiquitin-conjugating enzyme S; E2EPF; E2-EPF; E2-EPF5; EPF5; ubiquitin carrier protein S; ubiquitin conjugating enzyme E2S; ubiquitin-conjugating enzyme E2 S; ubiquitin-conjugating enzyme E2-24 kD; ubiquitin-conjugating enzyme E2-24 kDa; ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-protein ligase S
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.