Bethyl Laboratories
Rabbit anti-E2F1 Antibody Affinity Purified
Primary
Catalog #A300-765A
TargetE2F1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
E2F1 is a member of the E2F family of transcription factors. E2F is an activating member that binds DNA at the E2 recognition site in the promoters of genes involved in cell cycle regulation or DNA replication. E2F1 is regulated through its interaction with the pRb tumor suppressor.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 300 and 350
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetE2F1
Antigen Species
Human
Gene ID1869
Gene Symbol
E2F1
Gene Name
E2F transcription factor 1
Uniprot IDQ01094
Protein Name
Transcription factor E2F1
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to E2F1 immobilized on solid support.
The epitope recognized by A300-765A maps to a region between residue 300 and 350 of human E2F Transcription Factor 1 using the numbering given in entry NP_005216.1 (GeneID 1869).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A300-765A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-765A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-765AOpen
Citations / Publications
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