Rabbit anti-eIF6 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A303-030A maps to a region between residue 150 and 200 of human Eukaryotic Translation Initiation Factor 6 using the numbering given in entry NP_852133.1 (GeneID 3692).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to eIF6 immobilized on solid support.
The epitope recognized by A303-030A-T maps to a region between residue 150 and 200 of human Eukaryotic Translation Initiation Factor 6 using the numbering given in entry NP_852133.1 (GeneID 3692).
Additional Product Information
Eukaryotic translation initiation factor 6 (eIF6) is insoluble and found both in the nucleus and in the cytoplasm. eIF6 can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits.eIF6 binds to the fibronectin type III domains of ITGB4 in hemidesmosomes and may help link ITGB4 to the intermediate filament cytoskeleton [taken from NCBI Entrez Gene (Gene ID: 3692].
Alternate Names
b(2)gcn; B(2)GCN homolog; B4 integrin interactor; CAB; EIF3A; eIF-6; eukaryotic translation initiation factor 3A; eukaryotic translation initiation factor 6; ITGB4BP; p27 beta-4 integrin-binding protein; p27(BBP); p27BBP
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.