Rabbit anti-EML4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-909A maps to a region between residue 931 and 981of human echinoderm microtubule associated protein like 4 using the numbering given in entry NP_061936.2 (GeneID 27436).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EML4 immobilized on solid support.
The epitope recognized by A301-909A-T maps to a region between residue 931 and 981of human echinoderm microtubule associated protein like 4 using the numbering given in entry NP_061936.2 (GeneID 27436).
Additional Product Information
Echinoderm microtubule associated protein like 4 (EML4) has been identified as part of a gene fusion with the kinase domain of the anaplastic lymphoma receptor tyrosine kinase (ALK) in non-small cell lung cancer. EML4 associates with in vitro polymerized microtubules. In the cell, phosphorylated EML4 associates with the mitotic spindle and is essential for proliferation and microtubule network formation.
Alternate Names
C2orf2; echinoderm microtubule-associated protein-like 4; ELP120; EMAP-4; EMAPL4; restrictedly overexpressed proliferation-associated protein; ropp 120; ROPP120
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.