Rabbit anti-ERCC3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-337A-T maps to a region between residue 732 and 782 of human excision repair cross-complementing rodent repair deficiency, complementation group 3 using the numbering given in entry NP_000113.1 (GeneID 2071). Antibody was affinity purified using an epitope specific to ERCC3 immobilized on solid support.
The epitope recognized by A301-337A maps to a region between residue 732 and 782 of human excision repair cross-complementing rodent repair deficiency, complementation group 3 using the numbering given in entry NP_000113.1 (GeneID 2071).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
ERCC3 is a component of the core TFIIH basal transcription factor and functions as an ATP-dependent 3’-5’ DNA helicase. ERCC3 is involved in nucleotide excision repair and is the cause of xeroderma pigmentosum complementation group B (XPB), also known as xeroderma pigmentosum II (XP2). Additionally, it has been found to be the cuase of Cockayne syndrome, and trichothiodystrophy (TTD). XPB is an autosomal recessive disease characterized by skin photosensitivity and a predisposition to skin cancer, and neurological abnormalities. Cockayne syndrome and TTD are similarly characterized by photosensitivity and neural abnormalities.
Alternate Names
basic transcription factor 2 89 kDa subunit; BTF2; BTF2 p89; DNA excision repair protein ERCC-3; DNA repair helicase; DNA repair protein complementing XP-B cells; excision repair cross-complementation group 3; excision repair cross-complementing rodent repair deficiency, complementation group 3; general transcription and DNA repair factor IIH helicase subunit XPB; GTF2H; RAD25; TFIIH; TFIIH 89 kDa subunit; TFIIH basal transcription factor complex 89 kDa subunit; TFIIH basal transcription factor complex helicase XPB subunit; TFIIH p89; TFIIH subunit XPB; TTD2; xeroderma pigmentosum group B-complementing protein; xeroderma pigmentosum, complementation group B; XPB
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.