/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-FIH-1 Antibody
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Primary Antibodies  

Rabbit anti-FIH-1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

FIH-1

Reactivity:

Human

Applications:

IHC

,

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 299 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Zebrafish

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Country of Origin

USA

Additional Product Information

Factor inhibiting HIF-1 (FIH-1) hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). FIH-1 functions as an oxygen sensor, and under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. FIH-1 is involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NWT6].

55662

HIF1AN

hypoxia inducible factor 1 subunit alpha inhibitor

Q9NWT6

Hypoxia-inducible factor 1-alpha inhibitor

Alternate Names

factor inhibiting HIF1; factor inhibiting HIF-1; FIH1; FIH-1; hypoxia inducible factor 1 alpha subunit inhibitor; hypoxia-inducible factor 1-alpha inhibitor; hypoxia-inducible factor asparagine hydroxylase; peptide-aspartate beta-dioxygenase

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.