Rabbit anti-FOXK1 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A301-728A maps to a region between residue 683 and 733 of human forkhead box K1 using the numbering given in entry NP_001032242.1 (GeneID 221937).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to FOXK1 immobilized on solid support.
The epitope recognized by A301-728A-T maps to a region between residue 683 and 733 of human forkhead box K1 using the numbering given in entry NP_001032242.1 (GeneID 221937).
Additional Product Information
Forkhead box protein K1 (FOXK1) is a member of the human Forkhead-box (FOX) family of proteins. The Forkhead-box domain is a highly conserved DNA binding domain of about 100 amino acids found in proteins that function as transcriptional regulators. FOXK1 also bears an FHA (forkhead-associated) domain, a putative nuclear signaling domain that binds phospho-peptides. FOXK1 has been shown to play a role in the regulation of myogeneic progenitor cells and skeletal muscle regeneration.
Alternate Names
forkhead box protein K1; FOXK1L; MNF; myocyte nuclear factor
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.