Bethyl Laboratories
Rabbit anti-FOXO3a Recombinant Monoclonal Antibody [BLR140H]
Monoclonal Antibodies
Catalog #A700-140
TargetFOXO3a
HostRabbit
CloneBLR140H
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
Forkhead box protein 03A (FOXO3a) belongs to a large family of Forkhead proteins that possesses a winged-helix DNA-binding domain called Forkhead box (FOX). FOXO3a is a transcription factor that functions to induce apoptosis in the absence of survival factors and in response to oxidative stress. Under conditions of oxidative stress FOXO3a is acetylated and localized to the nucleus where it may act as a transcriptional repressor.
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR140H
Host
Rabbit
Immunogen
Between 475 and 525
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
40 µg/ml
Target Identity
TargetFOXO3a
Antigen Species
Human
Gene ID2309
Gene Symbol
FOXO3
Gene Name
forkhead box O3
Uniprot IDO43524
Protein Name
Forkhead box protein O3
Product Information
Applications
IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 450 and 500 of human Forkhead box protein O3 using the numbering given in entry NP_001446.1 (Gene ID 2309).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-140 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-140 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-140 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-140Open
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
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Full custom monoclonal development, from immunization through validation.
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Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
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