/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-gamma-H2AX Recombinant Monoclonal Antibody [BLR053F]
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Primary Antibodies  

Rabbit anti-gamma-H2AX Recombinant Monoclonal Antibody [BLR053F]

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (4)
Target:

gamma-H2AX

Reactivity:

Human

,

Mouse

Applications:

Flow Cyt

,

ICC

,

IHC

,

IHC-IF

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR053F

Format:

Whole IgG

Immunogen:

Surrounding Serine 140

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

100 µg (BSA-Free)

$127.00 $794.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
100 µg/ml 1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide


Request Formulation Change

Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a peptide surrounding phosphorylated serine 140 of human histone H2AX using the numbering given in entry NP_002096.1 (Gene ID 3014).

Country of Origin

USA

Additional Product Information

H2AX is a member of the histone H2A family. The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX may function to facilitate DNA repair, and recent studies have shown that H2AX is required for the maintenance of genomic stability. Gamma H2AX is the phosphorylated form of H2AX that results in response to DNA damage.

3014

H2AFX

H2A histone family member X

P16104

Histone H2AX

Alternate Names

H2A.X; H2A/X; H2AX; H2AX histone; histone H2A.x; histone H2AX

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.