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Primary Antibodies  

Rabbit anti-GFP Recombinant Monoclonal Antibody [BLRE04L]

bethyl
Bethyl Laboratories Catalog # A191-104
Validated
Target:
GFP
Applications:
ICC, IP, WB
Host:
Rabbit
Clonality:
Recombinant Monoclonal
Clone:
GFP
Format:
Whole IgG
Immunogen:
Green Fluorescent Protein
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Purified
100 µl (50+ tests)

A191-104
$482.00
Qty:
Bulk Request

Product Details

Specifications

Concentration
50 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Request Formulation Change
Production Procedures
Recombinant antibody was purified from cell culture supernatant.

Immunogen was a recombinant protein representing Green Fluorescent Protein (UniProt P42212).
Country of Origin
USA

Additional Product Information

Green fluorescent protein (GFP) and variants such as EGFP, YP, CFP, AcGFP, or BFP are widely used as fluorescent markers of gene expression and for determination of protein localization and motility in living cells.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.