Rabbit anti-GLOD4 Antibody Affinity Purified

Specifications

Production & Epitope

Antibody was affinity purified using an epitope specific to GLOD4 immobilized on solid support.

The epitope recognized by A305-663A maps to a region between residue 100 to 150 of human Glyoxalase domain-containing protein 4 using the numbering given in entry Q9HC38.1 (GeneID 51031).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).