Rabbit anti-GTF3C3/TFIIIC102 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-237A-T maps to a region between residue 100 and 150 of human general transcription factor 3C polypeptide 3 (Transcription factor IIIC 102 kDa subunit) using the numbering given in entry NP_036218.1 (GeneID 9330).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to GTF3C3/TFIIIC102 immobilized on solid support.
The epitope recognized by A301-237A maps to a region between residue 100 and 150 of human general transcription factor 3C polypeptide 3 (Transcription factor IIIC 102 kDa subunit) using the numbering given in entry NP_036218.1 (GeneID 9330).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
General transcription factor 3C polypeptide 3 (GTF3C3/TFIIIC102) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C3/TFIIIC102 appears to be important for stable complex formation and recruitment of RNA polymerase III by interacting with TFIIIB90, TBP, and TFIIIC63. GTF3C3/TFIIIC102 has been shown to also interact with DEDD and FLAME-3 proteins. This interaction may function to translocate GTF3C3/TFIIIC102 to the nucleus and regulate TFIIIC activity.
Alternate Names
general transcription factor 3C polypeptide 3; general transcription factor IIIC, polypeptide 3, 102kDa; TF3C-gamma; TFIIIC 102 kDa subunit; TFIIIC102; TFiiiC2-102; TFIIICgamma; Transcription factor IIIC 102 kDa subunit; transcription factor IIIC subunit gamma
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.