/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Haspin Antibody
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Primary Antibodies  

Rabbit anti-Haspin Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (3)
Target:

Haspin

Reactivity:

Human

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 300 and 350

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to Haspin immobilized on solid support.

The epitope recognized by A302-241A maps to a region between residue 300 and 350 of human serine/threonine-protein kinase Haspin (germ cell-specific gene 2) using the numbering given in entry NP_114171.2 (GeneID 83903).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to Haspin immobilized on solid support.

The epitope recognized by A302-241A-T maps to a region between residue 300 and 350 of human serine/threonine-protein kinase Haspin (germ cell-specific gene 2) using the numbering given in entry NP_114171.2 (GeneID 83903).

Country of Origin

USA

Additional Product Information

Hapsin is a serine-threonine protein kinase that was originally identified as a cDNA expressed specifically in mouse testicular germ cells. Haspin associates with the chromosomes, centrosomes, and spindle and is critical for proper chromosome alignment and segregation. Haspin is responsible for the phosphorylation of histone H3 at threonine 3.

83903

HASPIN

histone H3 associated protein kinase

Q8TF76

Serine/threonine-protein kinase haspin

Alternate Names

germ cell associated 2, haspin; germ cell-specific gene 2 protein; GSG2; haploid germ cell-specific nuclear protein kinase; H-haspin; serine/threonine-protein kinase haspin

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.