Bethyl Laboratories

Rabbit anti-HBS1L Antibody Affinity Purified

Primary
Catalog #A305-362A
TargetHBS1L
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
Detection of human HBS1L by western blot.
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Product Description

HBS1-like protein (HBS1L) is a member of the GTP-binding elongation factor family. It is expressed in multiple tissues with the highest expression in heart and skeletal muscl [taken from NCBI Entrez Gene (Gene ID: 10767)].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 25 and 75
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetHBS1L
Antigen Species
Human
Gene ID10767
Gene Symbol
HBS1L
Gene Name
HBS1 like translational GTPase
Uniprot IDQ9Y450
Protein Name
HBS1-like protein
Alternate Names
EF-1a  •  eRF3 family member  •  eRF3c  •  ERF3-similar protein  •  ERFS  •  HBS1  •  HBS1-like protein  •  Hsp70 subfamily B suppressor 1-like protein  •  HSPC276
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to HBS1L immobilized on solid support.

The epitope recognized by A305-362A maps to a region between residue 25 to 75 of human HBS1-like protein using the numbering given in entry Q9Y450.1 (GeneID 10767).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western Blot (WB)1:2000 - 1:10,000

Documents

Product DatasheetPDF · A305-362A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-362A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-362AOpen
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