/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-HOOK1 Antibody
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Primary Antibodies  

Rabbit anti-HOOK1 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

HOOK1

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 678 and 728

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

HOOK1 is required for spermatid differentiation. It is probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). It is a component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex) [taken from NCBI Entrez Gene (Gene ID: 51361)]. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UJC3].

51361

HOOK1

hook microtubule tethering protein 1

Q9UJC3

Protein Hook homolog 1

Alternate Names

hHK1; h-hook1; HK1; hook homolog 1; protein Hook homolog 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.