Rabbit anti-HOOK1 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A304-887A maps to a region between residue 678 to 728 of human Protein Hook homolog 1 using the numbering given in entry Q9UJC3.2 (GeneID 51361).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HOOK1 immobilized on solid support.
The epitope recognized by A304-887A-T maps to a region between residue 678 to 728 of human Protein Hook homolog 1 using the numbering given in entry Q9UJC3.2 (GeneID 51361).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
HOOK1 is required for spermatid differentiation. It is probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). It is a component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex) [taken from NCBI Entrez Gene (Gene ID: 51361)]. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UJC3].
Alternate Names
hHK1; h-hook1; HK1; hook homolog 1; protein Hook homolog 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.