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Rabbit anti-ICAM-1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
ICAM-1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 482 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to ICAM-1 immobilized on solid support.

The epitope recognized by A302-874A-T maps to a region between residue 467 and 517 of human Intercellular Adhesion Molecule 1 using the numbering given in entry NP_000192.2 (GeneID 3383).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to ICAM-1 immobilized on solid support.

The epitope recognized by A302-874A maps to a region between residue 467 and 517 of human Intercellular Adhesion Molecule 1 using the numbering given in entry NP_000192.2 (GeneID 3383).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Intercellular adhesion molecule 1 (ICAM-1) is a ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM-1 is also the cellular receptor for rhinovirus and coxsackievirus.

3383
ICAM1
intercellular adhesion molecule 1
P05362
Intercellular adhesion molecule 1

Alternate Names

BB2; CD antigen CD54; CD54; cell surface glycoprotein P3.58; epididymis secretory sperm binding protein; ICAM-1; intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor; P3.58

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.