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Rabbit anti-JMJD2A IHC Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
JMJD2A
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 350 and 400
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

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Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
White-tufted-ear marmoset, Crab-eating macaque, Gorilla, Chimpanzee, Orangutan
Antigen Species
Human
Stock Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to JMJD2A immobilized on solid support.

The epitope recognized by IHC-00188-T maps to a region between residues 350 and 400 of human Jumonji Domain Containing 2A using the numbering given in entry NP_055478.1 (GeneID 9682).

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Gene & Protein Information

JMJD2A is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. Some recently characterized members of the jmj family include JARID1A/RBP2, JARID1C, JMJD1A, JMJD1B, JMJD1C, JMJD2A, JMJD2C and JMJD2D. JMJD2A is a trimethylation-specific demethylase and functions as a transcriptional repressor. JMJD2A interacts with pRb and class one histone deacetylases to mediate repression of E2F-regulated promoters.

9682

KDM4A

lysine demethylase 4A

O75164

Lysine-specific demethylase 4A

Alternate Names

JHDM3A, jmjC domain-containing histone demethylation protein 3A, JMJD2, JMJD2A, jumonji C domain-containing histone demethylase 3A, jumonji domain containing 2, jumonji domain containing 2A, jumonji domain-containing protein 2A, lysine (K)-specific demethylase 4A, lysine-specific demethylase 4A, TDRD14A, tudor domain containing 14A

Applications

Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.