Bethyl Laboratories
Rabbit anti-Ki-67 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00395
TargetKi-67
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
Ki-67 is a marker of cell proliferation detected in cells that are in G1-, S-, G2-, and M-phase of the cell cycle and absent in cells in G0. Ki-67 is detected exclusively in the nucleus, and its detection by immunohistological methods has proven to have diagnostic and prognostic value in the study of a variety of human tumors. The specific cellular functions of Ki-67 have not been elucidated, but it is proposed to function in the organization and maintenance of the architecture of DNA and in the synthesis of ribosomes during mitosis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1800 and 1850
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetKi-67
Antigen Species
Human
Gene ID4288
Gene Symbol
MKI67
Gene Name
marker of proliferation Ki-67
Uniprot IDP46013
Protein Name
Antigen KI-67
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Ki-67 immobilized on solid support.
The epitope recognized by IHC-00395 maps to a region between residues 1800 and 1850 of human Ki-67 using the numbering given in entry NP_002408.2 (GeneID 4288).
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00395Open
Product DatasheetPDF · IHC-00395 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00395 · Lot 1 (current lot)Open
Citations / Publications
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Resources
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