Rabbit anti-KIF18A Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A301-079A-T maps to a region between residue 650 and 700 of human kinesin family member 18A using the numbering given in entry NP_112494.2 (GeneID 81930).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to KIF18A immobilized on solid support.
The epitope recognized by A301-079A maps to a region between residue 650 and 700 of human kinesin family member 18A using the numbering given in entry NP_112494.2 (GeneID 81930).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The kinesin superfamily of proteins consists of over forty KIF motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, chromosome congression, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. KIF18 has been demonstrated to be a microtubule depolymerase essential for chromosome alignment at the spindle equator.
Alternate Names
kinesin-like protein KIF18A; Marrow stromal KIF18A; MS-KIF18A; PPP1R99; protein phosphatase 1, regulatory subunit 99
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.