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Rabbit anti-Leukotriene A4 Hydrolase/LTA4H Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
Leukotriene A4 Hydrolase/LTA4H
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 561 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to Leukotriene A4 Hydrolase/LTA4H immobilized on solid support.

The epitope recognized by A305-006A maps to a region between residue 561 to 611 of human Leukotriene A-4 hydrolase using the numbering given in entry P09960.2 (GeneID 4048).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to Leukotriene A4 Hydrolase/LTA4H immobilized on solid support.

The epitope recognized by A305-006A-T maps to a region between residue 561 to 611 of human Leukotriene A-4 hydrolase using the numbering given in entry P09960.2 (GeneID 4048).
Country of Origin
USA

Additional Product Information

Leukotriene A4 Hydrolase/LTA4H is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD) [taken from NCBI Entrez Gene (Gene ID: 4048)].

4048
LTA4H
leukotriene A4 hydrolase
P09960
Leukotriene A-4 hydrolase

Alternate Names

Leukotriene A(4) hydrolase; leukotriene A-4 hydrolase; LTA-4 hydrolase; testicular secretory protein Li 27

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.