Rabbit anti-Leukotriene A4 Hydrolase/LTA4H Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A305-006A maps to a region between residue 561 to 611 of human Leukotriene A-4 hydrolase using the numbering given in entry P09960.2 (GeneID 4048).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Leukotriene A4 Hydrolase/LTA4H immobilized on solid support.
The epitope recognized by A305-006A-T maps to a region between residue 561 to 611 of human Leukotriene A-4 hydrolase using the numbering given in entry P09960.2 (GeneID 4048).
Additional Product Information
Leukotriene A4 Hydrolase/LTA4H is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD) [taken from NCBI Entrez Gene (Gene ID: 4048)].
Alternate Names
Leukotriene A(4) hydrolase; leukotriene A-4 hydrolase; LTA-4 hydrolase; testicular secretory protein Li 27
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.