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Rabbit anti-MCM5 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
MCM5
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to MCM5 immobilized on solid support.

The epitope recognized by A300-195A maps to a region between residues 1 and 50 of human Minichromosome Maintenance 5 using the numbering given in entry NP_006730.2 (GeneID 4174).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to MCM5 immobilized on solid support.

The epitope recognized by A300-195A-T maps to a region between residues 1 and 50 of human Minichromosome Maintenance 5 using the numbering given in entry NP_006730.2 (GeneID 4174).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM5 can form a subcomplex with MCM3. MCM5 also appears to play a role in transcription. MCM5 has been shown to interact with the signal transducer and activator of transcription 1 (STAT1) and demonstrated to be essential for STAT1 target gene transcription.

4174
MCM5
minichromosome maintenance complex component 5
P33992
DNA replication licensing factor MCM5

Alternate Names

CDC46; CDC46 homolog; DNA replication licensing factor MCM5; MCM5 minichromosome maintenance deficient 5, cell division cycle 46; MGORS8; minichromosome maintenance deficient 5 (cell division cycle 46); P1-CDC46

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.