Bethyl Laboratories
Rabbit anti-MEF2C Recombinant Monoclonal Antibody [BLR327M]
Primary
Catalog #A700-327
TargetMEF2C
HostRabbit
CloneBLR327M
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
MEF2C is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It also plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. MEF2C is crucial for normal neuronal development, distribution, and electrical activity in the neocortex. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q06413].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR327M
Host
Rabbit
Immunogen
between 425 and 475
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetMEF2C
Antigen Species
Human
Gene ID4208
Gene Symbol
MEF2C
Gene Name
myocyte enhancer factor 2C
Uniprot IDQ06413
Protein Name
Myocyte-specific enhancer factor 2C
Product Information
Applications
IHC, IP, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 425 and 475 of human Myocyte-specific enhancer factor 2C using the numbering given in entry NP_002388.2 (Gene ID 4208).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1,000
Immunoprecipitation (IP)6 µl/mg lysate
Documents
Product DatasheetPDF · A700-327 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-327 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-327Open
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