Rabbit anti-Metnase Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-615A-T maps to a region between residue 225 and 275 of human SET Domain and Mariner Transposase Fusion Gene-Containing Protein using the numbering given in entry BAD96454.1 (GeneID 6419).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Metnase immobilized on solid support.
The epitope recognized by A303-615A maps to a region between residue 225 and 275 of human SET Domain and Mariner Transposase Fusion Gene-Containing Protein using the numbering given in entry BAD96454.1 (GeneID 6419).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Metnase is also known as Histone-lysine N-methyltransferase SETMAR. Metnase is a histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3, 2 specific tags for epigenetic transcriptional activation. Metanase specifically mediates dimethylation of H3 'Lys-36', and has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element. Metnase possessess DNA nicking activity and in vivo end joining activity, and it may mediate genomic integration of foreign DNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q53H47].
Alternate Names
Histone-lysine N-methyltransferase; histone-lysine N-methyltransferase SETMAR; Mar1; METNASE; SET domain and mariner transposase fusion gene-containing protein; SET domain and mariner transposase fusion protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.