Rabbit anti-NDUFB6 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A305-436A maps to a region between residue 78 to 128 of human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 using the numbering given in entry O95139.3 (GeneID 4712).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NDUFB6 immobilized on solid support.
The epitope recognized by A305-436A-T maps to a region between residue 78 to 128 of human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 using the numbering given in entry O95139.3 (GeneID 4712).
Additional Product Information
NDUFB6 is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. NDUFB6 has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone [taken from NCBI Entrez Gene (Gene ID: 4712)].
Alternate Names
B17; CI; CI-B17; complex I, mitochondrial respiratory chain, B17 subunit; complex I-B17; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa; NADH dehydrogenase ubiquinone 1 beta subcomplex subunit 6; NADH-ubiquinone oxidoreductase B17 subunit; NADH-ubiquinone oxidoreductase beta subunit, 6
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.