Rabbit anti-NF2 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A300-578A maps to a region between residue 550 and the C-terminus (residue 595) of human Neurofibromin 2 using the numbering given in entry NP_000259.1 (GeneID 4771). This epitope is common to NF2 isoforms 1 and 9 (Mer162).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NF2 immobilized on solid support.
The epitope recognized by A300-578A-T maps to a region between residue 550 and the C-terminus (residue 595) of human Neurofibromin 2 using the numbering given in entry NP_000259.1 (GeneID 4771). This epitope is common to NF2 isoforms 1 and 9 (Mer162).
Additional Product Information
Mutations in NF2 (neurofibromin 2) are associated with neurofibromatosis type II which is characterized by Schwann cell tumors (schwannomas) which are benign slow-growing tumors that are resistant to chemotherapeutic intervention. NF2 is a membrane/cytoskeletal associated protein that has been classified as a novel type of tumor suppressor due to its function in membrane organization versus a classic tumor suppressor function that regulates cell cycle machinery.
Alternate Names
ACN; BANF; merlin; moesin-ezrin-radixin like; moesin-ezrin-radixin-like protein; moesin-ezrin-radizin-like protein; neurofibromin 2 (bilateral acoustic neuroma); Neurofibromin-2; SCH; schwannomerlin; schwannomin
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.