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Primary Antibodies  

Rabbit anti-NFAT1 Antibody

Bethyl Laboratories®
Bethyl Laboratories®

Catalog #

Validated
Target:

NFAT1

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 875 and 925

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Additional Product Information

Nuclear factor of activated T cells 1(NFAT1) is a member of the NFAT family of transcription factors which includes NFAT1 (NFATp/NFATc2), NFAT2 (NFATc/NFATc1), NFAT3 (NFATc4), and NFAT4 (NFATc3/NFATx). Different members of the NFAT family are expressed in a distinct but overlapping set of cell types. NFAT1 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFAT1 is a member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFAT1 and an inducible nuclear component NFAT2 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q13469].

4773

NFATC2

nuclear factor of activated T cells 2

Q13469

Nuclear factor of activated T-cells, cytoplasmic 2

Alternate Names

NFAT pre-existing subunit; NFAT transcription complex, preexisting component; NFAT1; NFATc2; NF-ATc2; NFATP; NF-ATp; nuclear factor of activated T-cells, cytoplasmic 2; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2; nuclear factor of activated T-cells, preexisting component; preexisting nuclear factor of activated T-cells 2; T cell transcription factor NFAT1; T-cell transcription factor NFAT1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.