Rabbit anti-NFATc3 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A303-572A maps to a region between residue 1025 and 1075 of human Nuclear Factor of Activated T-cells, Cytoplasmic, Calcineurin-dependent 3 using the numbering given in entry NP_775188.1 (GeneID 4775).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NFATc3 immobilized on solid support.
The epitope recognized by A303-572A-T maps to a region between residue 1025 and 1075 of human Nuclear Factor of Activated T-cells, Cytoplasmic, Calcineurin-dependent 3 using the numbering given in entry NP_775188.1 (GeneID 4775).
Additional Product Information
Nuclear factor of activated T cells, cytoplasmic 1(NFATc3) is a member of the NFAT family of transcription factors which includes NFAT1 (NFATp/NFATc2), NFAT2 (NFATc/NFATc1), NFAT3 (NFATc4), and NFAT4 (NFATc3/NFATx). Different members of the NFAT family are expressed in a distinct but overlapping set of cell types. NFATc3 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of IL-2.
Alternate Names
NFAT4; NF-AT4; NFATc3; NF-ATc3; NFATX; nuclear factor of activated T-cells c3 isoform IE-Xa; nuclear factor of activated T-cells, cytoplasmic 3; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; T cell transcription factor NFAT4; T-cell transcription factor NFAT4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.