Rabbit anti-NFIC Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Nuclear factor I/C (NFIC) recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08651].
Alternate Names
CCAAT-box-binding transcription factor; CTF; CTF5; NF1-C; NFI; NF-I; NF-I/C; NFI-C; nuclear factor 1 C-type; Nuclear factor 1/C; Nuclear factor I/C; nuclear factor I/C (CCAAT-binding transcription factor); TGGCA-binding protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.