Bethyl Laboratories
Rabbit anti-NIPBL Antibody Affinity Purified
Primary
Catalog #A301-779A
TargetNIPBL
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
Image 1 of 2
local_shipping
Order within 5 hours and 33 minutes for shipment by today
Product Description
The NIPBL (Nipped B-like) gene was originally identified as a gene mutated in individuals with Cornelia de Lange syndrome (CdLS), a heterogenous developmental disorder associated with multiple anatomical malformations. The NIPBL protein product is also referred to as delangin. It has substantial homology to the D. melanogaster Nipped-B protein which is related to a family of chromosomal adherins that plays a role in sister chromatid adhesion, chromosome condensation, and DNA repair. A study of metaphase spreads from individuals positive and negative for NIPBL mutations revealed an association between NIPBL mutations and precocious sister chromatid separation. This result suggests a function for NIPBL that is similar to its homolog in drosophila.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1025 and 1075
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNIPBL
Antigen Species
Human
Gene ID25836
Gene Symbol
NIPBL
Gene Name
NIPBL, cohesin loading factor
Uniprot IDQ6KC79
Protein Name
Nipped-B-like protein
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NIPBL immobilized on solid support.
The epitope recognized by A301-779A maps to a region between residue 1025 and 1075 of human Nipped-B-like using the numbering given in entry NP_597677.2 (GeneID 25836).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Western Blot (WB)1:10,000 - 1:25,000 (3-8% Tris-acetate gel recommended)
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-779A · Lot 5 (current lot)Open
Product DatasheetPDF · A301-779A · Lot 5 (current lot)Open
Safety Data SheetPDF · A301-779AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1