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Rabbit anti-Nucleolin (NCL) Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
Nucleolin/NCL
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 550 and C-term
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

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Product Details

Specifications
Verified Reactivity
Mouse, Human
Presumed Reactivity
Orangutan
Antigen Species
Human
Stock Concentration
20 µg/ml
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to Nucleolin (NCL) immobilized on solid support.

The epitope recognized by A300-711A-T maps to a region between residue 550 and the C-terminus (residue 710) of human Nucleolin using the numbering given in entry NP_005372.2 (GeneID 4691).

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Gene & Protein Information

Nucleolin (NCL) is a major nucleolar phosphoprotein that is involved in pre-RNA transcription and the synthesis and maturation of ribosomes. NCL can bind to histone H1 to induce chromatin condensation and may play an additional role in transcriptional elongation.

4691

NCL

nucleolin

P19338

Nucleolin

Alternate Names

C23, Nsr1, nucleolin, Protein C23

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Citations (5)