/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Nucleolin/NCL Antibody
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Primary Antibodies  

Rabbit anti-Nucleolin (NCL) Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (22)
Target:

Nucleolin/NCL

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IHC-IF

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 660 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to Nucleolin (NCL) immobilized on solid support.

The epitope recognized by A300-711A maps to a region between residue 550 and the C-terminus (residue 710) of human Nucleolin using the numbering given in entry NP_005372.2 (GeneID 4691).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to Nucleolin (NCL) immobilized on solid support.

The epitope recognized by A300-711A-T maps to a region between residue 550 and the C-terminus (residue 710) of human Nucleolin using the numbering given in entry NP_005372.2 (GeneID 4691).

Country of Origin

USA

Additional Product Information

Nucleolin (NCL) is a major nucleolar phosphoprotein that is involved in pre-RNA transcription and the synthesis and maturation of ribosomes. NCL can bind to histone H1 to induce chromatin condensation and may play an additional role in transcriptional elongation.

4691

NCL

nucleolin

P19338

Nucleolin

Alternate Names

C23; Nsr1; nucleolin; Protein C23

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.