Bethyl Laboratories
Rabbit anti-NUMA Antibody Affinity Purified
Primary
Catalog #A301-510A
TargetNUMA
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
The nuclear mitotic apparatus protein (NuMA) is a critical component of the mitotic spindle that is responsible for organizing minus-ends of microtubules at spindle poles. NUMA is regulated by phosphorylation and associates with dynein and dynactin in its phosphorylated form. Dephosphorylation of NUMA in anaphase results in the release from dynein and dynactin to allow spindle disassembly. NUMA, in association with dynein and dynactin, also interacts with 4.1R in a complex of spindle pole organizing proteins. Other NUMA interacting proteins include GAS41, tankyrase, and Rae1. These interactions indicate a broader role for NUMA function in the nucleus.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2065 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetNUMA
Antigen Species
Human
Gene ID4926
Gene Symbol
NUMA1
Gene Name
nuclear mitotic apparatus protein 1
Uniprot IDQ14980
Protein Name
Nuclear mitotic apparatus protein 1
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NUMA immobilized on solid support.
The epitope recognized by A301-510A maps to a region between residue 2065 and 2115 of human nuclear mitotic apparatus protein 1 using the numbering given in entry NP_006176.2 (GeneID 4926).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A301-510A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-510A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-510AOpen
Citations / Publications
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