Rabbit anti-OSR1 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A301-578A-T maps to a region between residue 325 and 375 of human oxidative-stress responsive 1 using the numbering given in entry NP_005100.1 (GeneID 9943).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to OSR1 immobilized on solid support.
The epitope recognized by A301-578A maps to a region between residue 325 and 375 of human oxidative-stress responsive 1 using the numbering given in entry NP_005100.1 (GeneID 9943).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Oxidative stress responsive 1 (OSR1) is a STE20-related kinase involved in ion transport and fluid and ion homeostasis. OSR1 interacts with SPAK (STE20/SPS1-reated proline/alanine-rich kinase) and together are activated by the upstream kinase WNK1 [with no K (lysine) protein kinase-1]. Activation of OSR1 and SPAK by WNK1 results in the phosphorylation and activation of NKCC1 (Na+-K+-2Cl- co-transporter 1) for the control of cell volume and blood pressure.
Alternate Names
OSR1; oxidative stress-responsive 1 protein; serine/threonine-protein kinase OSR1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.