/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-p300 Antibody
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Primary Antibodies  

Rabbit anti-p300 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (27)
Target:

p300

Reactivity:

Human

Applications:

ChIP

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 950 and 1000

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to p300 immobilized on solid support.

The epitope recognized by A300-358A maps to a region between residues 950 and 1000 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to p300 immobilized on solid support.

The epitope recognized by A300-358A-T maps to a region between residues 950 and 1000 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033).

Country of Origin

USA

Additional Product Information

By sequence similarity p300 is related to CBP (CREB-binding protein) and functions as a histone acetyltransferase that can regulate transcription by influencing chromatin organization. P300 has been demonstrated to bind to phosphorylated CREB to mediate cAMP-gene regulation. P300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha).

2033

EP300

E1A binding protein p300

Q09472

Histone acetyltransferase p300

Alternate Names

E1A-associated protein p300; E1A-binding protein, 300kD; histone acetyltransferase p300; histone butyryltransferase p300; histone crotonyltransferase p300; KAT3B; p300; p300 HAT; protein propionyltransferase p300; RSTS2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.