Bethyl Laboratories
Rabbit anti-Paf1 Antibody Affinity Purified
Primary
Catalog #A300-173A
TargetPaf1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP
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Product Description
Paf1 is a component of the Paf1 complex that was originally identified in yeast as an RNA-polymerase II (RNAP II)-associated complex. The Paf1 complex functions in multiple aspects of gene expression. Paf1 has been shown to play roles in transcript elongation, histone H2B monoubiquitination, H3 methylation, chromatin remodeling, mRNA 3’-end processing, and the coupling of transcriptional and posttranscriptional events.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 481 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPaf1
Antigen Species
Human
Gene ID54623
Gene Symbol
PAF1
Gene Name
PAF1 homolog, Paf1/RNA polymerase II complex component
Uniprot IDQ8N7H5
Protein Name
RNA polymerase II-associated factor 1 homolog
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Paf1 immobilized on solid support.
The epitope recognized by A300-173A maps to a region between residues 475 and 525 of human RNA Polymerase Associated Factor using the numbering given in TrEMBL entry Q9NUU9 (GeneID 54623).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A300-173A · Lot 3 (current lot)Open
Product DatasheetPDF · A300-173A · Lot 3 (current lot)Open
Safety Data SheetPDF · A300-173AOpen
Citations / Publications
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