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Rabbit anti-PAN2/USP52 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
PAN2/USP52
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Mouse, Human
Presumed Reactivity
Rat
Antigen Species
Human
Stock Concentration
100 µg/ml
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to PAN2/USP52 immobilized on solid support.

The epitope recognized by A304-882A-T maps to a region between residue 1 to 50 of human PAB-dependent poly using the numbering given in entry Q504Q3.3 (GeneID 9924).

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Gene & Protein Information

PAB-dependent poly(A)-specific ribonuclease subunit PAN2 is the catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q504Q3].

9924

PAN2

poly(A) specific ribonuclease subunit PAN2

Q504Q3

PAB-dependent poly(A)-specific ribonuclease subuni

Alternate Names

inactive ubiquitin carboxyl-terminal hydrolase 52, PAB1P-dependent poly(A)-nuclease, PAB1P-dependent poly(A)-specific ribonuclease, PAB-dependent poly(A)-specific ribonuclease subunit 2, PAB-dependent poly(A)-specific ribonuclease subunit PAN2, PABP-dependent poly(A) nuclease 2, PAN deadenylation complex catalytic subunit 2, PAN deadenylation complex subunit 2, PAN2 poly(A) specific ribonuclease subunit homolog, PAN2 polyA specific ribonuclease subunit, PAN2-PAN3 deadenylation complex catalytic subunit PAN2, poly(A)-nuclease deadenylation complex subunit 2, ubiquitin specific peptidase 52, ubiquitin specific protease 52, USP52

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.