Rabbit anti-PAP-gamma Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Poly(A) polymerase gamma (PAP-gamma) is a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. PAP-gamma is exclusively localized in the nucleus and exhibits nonspecific as well as CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity [taken from NCBI Entrez Gene (GeneID: 64895)].
Alternate Names
neo-PAP; neo-poly(A) polymerase; nuclear poly(A) polymerase gamma; PAP-gamma; poly(A) polymerase gamma; polynucleotide adenylyltransferase gamma; signal recognition particle RNA-adenylating enzyme; SRP RNA 3' adenylating enzyme/pap2; SRP RNA 3'-adenylating enzyme; SRP RNA-adenylating enzyme
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.