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Rabbit anti-PAPOLA Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
PAPOLA
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 600 and 650
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

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Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to PAPOLA immobilized on solid support.

The epitope recognized by A301-009A-T maps to a region between residue 600 and 650 of human poly(A) polymerase alpha using the numbering given in entry NP_116021.2 (GeneID 10914).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

Poly(A) polymerase alpha (PAPOLA) is the polymerase responsible for catalyzing the addition of adenosine to the 3’end of pre-mRNAs for the synthesis of the 3’-end poly(A) tail. PAPOLA associates with the cleavage and polyadenylation specificity factor (CPSF) and Fip1 to perform this function.

10914

PAPOLA

poly(A) polymerase alpha

P51003

Poly(A) polymerase alpha

Alternate Names

PAP, PAP-alpha, poly(A) polymerase alpha, polynucleotide adenylyltransferase alpha

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE.