Rabbit anti-PAPOLA Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Poly(A) polymerase alpha (PAPOLA) is the polymerase responsible for catalyzing the addition of adenosine to the 3’end of pre-mRNAs for the synthesis of the 3’-end poly(A) tail. PAPOLA associates with the cleavage and polyadenylation specificity factor (CPSF) and Fip1 to perform this function.
Alternate Names
PAP; PAP-alpha; poly(A) polymerase alpha; polynucleotide adenylyltransferase alpha
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.