Rabbit anti-PDE4D Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A302-745A maps to a region between residue 759 and 809 of human Phosphodiesterase 4D using the numbering given in entry NP_001098101.1 (GeneID 5144).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PDE4D immobilized on solid support.
The epitope recognized by A302-745A-T maps to a region between residue 759 and 809 of human Phosphodiesterase 4D using the numbering given in entry NP_001098101.1 (GeneID 5144).
Additional Product Information
PDE4D (cAMP-specific 3’,5’-cyclic phosphodiesterase 4D) is one of four mammalian counterparts to the fruit fly 'dunce' gene. PDE4D has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types [taken from NCBI Entrez Gene (GeneID: 5144)].
Alternate Names
ACRDYS2; cAMP-specific 3',5'-cyclic phosphodiesterase 4D; cAMP-specific phosphodiesterase PDE4D6; DPDE3; HSPDE4D; PDE43; PDE4DN2; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila); STRK1; testicular tissue protein Li 136
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.