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Rabbit anti-PDE4D Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
PDE4D
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 759 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to PDE4D immobilized on solid support.

The epitope recognized by A302-745A maps to a region between residue 759 and 809 of human Phosphodiesterase 4D using the numbering given in entry NP_001098101.1 (GeneID 5144).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to PDE4D immobilized on solid support.

The epitope recognized by A302-745A-T maps to a region between residue 759 and 809 of human Phosphodiesterase 4D using the numbering given in entry NP_001098101.1 (GeneID 5144).
Country of Origin
USA

Additional Product Information

PDE4D (cAMP-specific 3’,5’-cyclic phosphodiesterase 4D) is one of four mammalian counterparts to the fruit fly 'dunce' gene. PDE4D has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types [taken from NCBI Entrez Gene (GeneID: 5144)].

5144
PDE4D
phosphodiesterase 4D
Q08499
cAMP-specific 3',5'-cyclic phosphodiesterase 4D

Alternate Names

ACRDYS2; cAMP-specific 3',5'-cyclic phosphodiesterase 4D; cAMP-specific phosphodiesterase PDE4D6; DPDE3; HSPDE4D; PDE43; PDE4DN2; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila); STRK1; testicular tissue protein Li 136

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.