Rabbit anti-PFKP Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-284A-T maps to a region between residue 375 to 425 of human Phosphofructokinase, Platelet using the numbering given in entry NP_002618.1 (GeneID 5214).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PFKP immobilized on solid support.
The epitope recognized by A304-284A maps to a region between residue 375 to 425 of human Phosphofructokinase, Platelet using the numbering given in entry NP_002618.1 (GeneID 5214).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Phosphofructokinase 1 catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q01813].
Alternate Names
6-phosphofructokinase type C; 6-phosphofructokinase, platelet type; ATP-dependent 6-phosphofructokinase, platelet type; ATP-PFK; PFK-C; PFKF; PFK-P; phosphofructo-1-kinase isozyme C; phosphofructokinase 1; phosphohexokinase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.