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Rabbit anti-Phospho MCM2 (S108) Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
MCM2 Phospho (S108)
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Surrounding serine 108
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $494.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using a synthetic peptide representing phosphorylation at Serine 108 and surrounding residues of MCM2 immobilized on solid support.

Immunogen A300-094A was a synthetic phosphorylated peptide, which represented a portion of human Minichromosomal Maintenance Deficient 2 surrounding Serine 108 according to the numbering given in entry NP_0045117.2 (GeneID 4171).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using a synthetic peptide representing phosphorylation at Serine 108 and surrounding residues of MCM2 immobilized on solid support.

Immunogen for A300-094A-T was a synthetic phosphorylated peptide, which represented a portion of human Minichromosomal Maintenance Deficient 2 surrounding Serine 108 according to the numbering given in entry NP_0045117.2 (GeneID 4171).
Country of Origin
USA

Additional Product Information

The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM2 interacts with the core MCM complex (MCM4, 6, and 7) to regulate its helicase activity.

4171
MCM2
minichromosome maintenance complex component 2
P49736
DNA replication licensing factor MCM2

Alternate Names

BM28; CCNL1; cdc19; CDCL1; cell devision cycle-like 1; cyclin-like 1; D3S3194; DFNA70; DNA replication licensing factor MCM2; minichromosome maintenance deficient 2 (mitotin); minichromosome maintenance protein 2 homolog; MITOTIN; nuclear protein BM28

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).