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Rabbit anti-Phospho SMC3 (S1083) Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
SMC3 Phospho (S1083)
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Surrounding serine 1083
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $494.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse, Rat
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to SMC3, Phospho (S1083) immobilized on solid support.

The epitope recognized by A304-637A maps to a region between residue The epitope recognized by A304-637A maps to a region surrounding residue 1083 when Serine 1083 is phosphorylated of human Structural Maintenance of Chromosomes 3 using the numbering given in SwissProt entry Q9UQE7 (GeneID 9126). of human Structural Maintenance of Chromosome 3 using the numbering given in entry NP_005436.1 (GeneID 9126).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

SMC3 (structural maintenance of chromosomes 3) is a component of the cohesin complex which is critical for precise duplication and segregation of chromosomes. SMC3 heterodimerizes with SMC1 and associates with two non-SMC subunits, Rad21/Scc1 and Scc3, to form the cohesin complex.

9126
SMC3
structural maintenance of chromosomes 3
Q9UQE7
Structural maintenance of chromosomes protein 3

Alternate Names

BAM; Bamacan; basement membrane-associated chondroitin proteoglycan; BMH; CDLS3; Chondroitin sulfate proteoglycan 6; chondroitin sulfate proteoglycan 6 (bamacan); chromosome-associated polypeptide; CSPG6; HCAP; SMC protein 3; SMC-3; SMC3L1; structural maintenance of chromosomes protein 3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.