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Rabbit anti-Phospho ZAP70 (Y493/Y494) Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
ZAP70 Phospho (Y493/Y494)
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
surrounding tyrosine 493/494
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

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Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
Mouse
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to Phospho ZAP70 (Y493/Y494) immobilized on solid support.

The epitope recognized by A302-009A-T maps to a region including tyrosines 493 and 494 (when they are phosphorylated) of human zeta-chain (TCR) associated protein kinase 70kDa using the numbering given in entry NP_001070.2 (GeneID 7535).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

ZAP-70 (zeta associated protein 70) is a tyrosine kinase of the Src family of tyrosine kinases. It was initially identified as a partner of the zeta chain of the CD3 complex associated to the T-cell receptor on T-lymphocytes. ZAP-70 shares homology with spleen-tyrosine kinase (Syk), a kinase involved in B-cell activation. Both ZAP-70 and Syk have been shown to be crucial to B- and T-lymphocyte development. In T-cells ZAP-70 functions to transduce the signaling cascade activated by T-cell receptor engagement and activation.

7535

ZAP70

zeta chain of T cell receptor associated protein k

P43403

Tyrosine-protein kinase ZAP-70

Alternate Names

70 kDa zeta-associated protein, 70 kDa zeta-chain associated protein, ADMIO2, IMD48, SRK, STCD, STD, syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, TZK, ZAP-70, zeta chain of T cell receptor associated protein kinase 70kDa, zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE.