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Rabbit anti-PIP1/WDR84 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
PIP1/WDR84
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 342 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to PIP1/WDR84 immobilized on solid support.

The epitope recognized by A301-550A maps to a region between residue 342 and 392 of human PAK/PLC-interacting protein 1 (WD repeat protein 84) using the numbering given in entry NP_060376.2 (GeneID 55003).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to PIP1/WDR84 immobilized on solid support.

The epitope recognized by A301-550A-T maps to a region between residue 342 and 392 of human PAK/PLC-interacting protein 1 (WD repeat protein 84) using the numbering given in entry NP_060376.2 (GeneID 55003).
Country of Origin
USA

Additional Product Information

PIP1/WDR84 (PAK1IP1) was identified as a p21-activated protein kinase (PAK) interacting protein that functions as a negative regulator of PAK activity and PAK signaling pathways. PIP1/WDR84 (PAK1IP1) contains 5 G beta-like WD repeats. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation.

55003
PAK1IP1
PAK1 interacting protein 1
Q9NWT1
p21-activated protein kinase-interacting protein 1

Alternate Names

bA421M1.5; hPIP1; MAK11; p21-activated protein kinase-interacting protein 1; PAK/PLC-interacting protein 1; PAK1-interacting protein 1; PIP1; WD repeat-containing protein 84; WDR84

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.