Rabbit anti-PKC-alpha Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A302-446A maps to a region between residue 622 and 672 of human protein kinase C, alpha using the numbering given in entry NP_002728.1 (GeneID 5578).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PKC-alpha immobilized on solid support.
The epitope recognized by A302-446A-T maps to a region between residue 622 and 672 of human protein kinase C, alpha using the numbering given in entry NP_002728.1 (GeneID 5578).
Additional Product Information
Protein kinase C alpha (PKC-alpha) is a member of the PKC family of serine-threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. PKC-alpha has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control [taken from NCBI Entrez Gene (GeneID: 5578)].
Alternate Names
AAG6; aging-associated gene 6; PKCA; PKC-A; PKCalpha; PKC-alpha; PKCI+/-; PRKACA; protein kinase C alpha type
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.