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Rabbit anti-PKC-alpha Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
PKC-alpha
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 622 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Bovine, Rat
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to PKC-alpha immobilized on solid support.

The epitope recognized by A302-446A maps to a region between residue 622 and 672 of human protein kinase C, alpha using the numbering given in entry NP_002728.1 (GeneID 5578).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to PKC-alpha immobilized on solid support.

The epitope recognized by A302-446A-T maps to a region between residue 622 and 672 of human protein kinase C, alpha using the numbering given in entry NP_002728.1 (GeneID 5578).
Country of Origin
USA

Additional Product Information

Protein kinase C alpha (PKC-alpha) is a member of the PKC family of serine-threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. PKC-alpha has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control [taken from NCBI Entrez Gene (GeneID: 5578)].

5578
PRKCA
protein kinase C alpha
P17252
Protein kinase C alpha type

Alternate Names

AAG6; aging-associated gene 6; PKCA; PKC-A; PKCalpha; PKC-alpha; PKCI+/-; PRKACA; protein kinase C alpha type

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.