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Rabbit anti-POLR1A/RPA194 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
POLR1A/RPA194
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1350 and 1400
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to POLR1A/RPA194 immobilized on solid support.

The epitope recognized by A304-845A-T maps to a region between residue 1350 to 1400 of human DNA-directed RNA polymerase I subunit RPA1 using the numbering given in entry O95602.2 (GeneID 25885).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to POLR1A/RPA194 immobilized on solid support.

The epitope recognized by A304-845A maps to a region between residue 1350 to 1400 of human DNA-directed RNA polymerase I subunit RPA1 using the numbering given in entry O95602.2 (GeneID 25885).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

POLR1A is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It is the largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O95602].

25885
POLR1A
RNA polymerase I subunit A
O95602
DNA-directed RNA polymerase I subunit RPA1

Alternate Names

A190; AFDCIN; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A; DNA-directed RNA polymerase I subunit A1; DNA-directed RNA polymerase I subunit RPA1; polymerase (RNA) I polypeptide A, 194kDa; polymerase (RNA) I subunit A; RNA polymerase I 194 kDa subunit; RNA polymerase I subunit A1; RPA1; RPA194; RPO14; RPO1-4

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.