Rabbit anti-PPA2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-539A maps to a region between residue 284 to 334 of human Inorganic pyrophosphatase 2, mitochondrial using the numbering given in entry Q9H2U2.2 (GeneID 27068).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PPA2 immobilized on solid support.
The epitope recognized by A305-539A-T maps to a region between residue 284 to 334 of human Inorganic pyrophosphatase 2, mitochondrial using the numbering given in entry Q9H2U2.2 (GeneID 27068).
Additional Product Information
PPA2 is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells [taken from NCBI Entrez Gene (Gene ID: 27068)].
Alternate Names
HSPC124; inorganic pyrophosphatase 2, mitochondrial; PPase 2; pyrophosphatase SID6-306; pyrophosphate phospho-hydrolase 2; SCFAI; SCFI; SID6-306
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.