/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-Pragmin Antibody
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Primary Antibodies  

Rabbit anti-Pragmin Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (4)
Target:

Pragmin

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 275 and 325

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

Pragmin is also known as SGK223 (sugen kinase 223). The rat homolog was originally identified in a yeast two-hybrid screen for Rnd2 effector proteins expressed in neural cells. Rat Pragmin was found to stimulate RhoA activity for the regulation of neurite outgrowth.

157285

PRAG1

PEAK1 related, kinase-activating pseudokinase 1

Q86YV5

Tyrosine-protein kinase SgK223

Alternate Names

homolog of rat pragma of Rnd2; inactive tyrosine-protein kinase PRAG1; NACK; PEAK family member 2; PEAK1-related kinase-activating pseudokinase 1; PEAK2; PRAGMIN; Pragmin, RND2 effector protein; SGK223; sugen kinase 223; tyrosine-protein kinase PRAG1; tyrosine-protein kinase SgK223

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.